IL6基因内含子3多态性与仔猪腹泻、生长和公猪繁殖性状的关联分析

doi:10.11843/j.issn.0366-6964.2017.09.012

Abstract

Abstract:

In order to discover new molecular marker related to piglet diarrhea, growth and boar reproduction traits, the polymorphism of porcine IL6 gene was investigated and the association analysis was performed in different pig breeds. The DNA fragment of IL6 gene was amplified and sequenced. A SNP (g.1704674C>T), which could alter the recognition site of HpaⅡ, was detected in intron 3 of IL6. The SNP was genotyped by PCR-RFLP in 281 Min piglets, 182 Landrace piglets, 215 Duroc boars and 69 Yorkshire boars. Its association with piglet diarrhea, growth and boar reproduction traits was analyzed. The expression of IL6 was detected in PBMC stimulated with LPS by real-time PCR. The results showed that Min piglets with CC genotype had significantly higher diarrhea index than TT genotype piglets (P<0.05). The 14, 21, 28, 35-day body weight and ADG of Min piglets with CC genotype were significantly lower than that of TC genotype (P<0.05), and the 21-day body weight of Min piglets was also significantly lower than that of piglets with TT genotype (P<0.05). In Landrace piglets, birth weight of piglets with TT genotype was significantly higher than that of piglets with CC genotype (P<0.05), and the 7-day body weight was significantly higher than that of individuals with TC genotype (P<0.05). In Duroc boars, the ejaculate volume of boars with TT and TC genotype was significantly higher than that of boars with CC genotype (P<0.05). In Yorkshire boar, the sperm density of boars with CC genotype was significantly higher than that of boars with TC genotype (P<0.05). At 12 and 24 h after LPS stimulation, the expression of IL6 gene was significantly increased in PBMC (P<0.01 or P<0.05). Therefore, it can be concluded IL6 g.1704674C> T had effect on piglet diarrhea, growth and boar reproduction traits, and which may be a potential molecular marker in pig breeding.

CLC Number:

S813.1

ZHA An-dong, XING Gui-ling, GAO Xiao-wen, ZHANG Long, DI Sheng-wei, WANG Xi-biao, NIU Bu-yue. Association Analysis between IL6 Gene Intron 3 Polymorphism and Piglet Diarrhea, Growth and Boar Reproduction Traits[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(9): 1674-1682.

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References

[1] WEISSENBACH J, CHERNAJOVSKY Y, ZEEVI M, et al. Two interferon mRNAs in human fibroblasts:in vitro translation and Escherichia coli cloning studies[J]. Proc Natl Acad Sci U S A, 1980, 77(12):7152-7156.
[2] HIRANO T, YASUKAWA K, HARADA H, et al. Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin[J]. Nature, 1986, 324(6092):73-76.
[3] 蓝天. 白细胞介素6及下游JAK/STAT3信号通路在前列腺相关疾病中的作用及机制研究[D]. 西安:第四军医大学, 2011.
LAN T. The functions and mechanism of interleukin-6 and downstream JAK/STAT3 signaling pathway in prostatic diseases[D]. Xi'an:Medical University of the Air Force, 2011. (in Chinese)
[4] 仇小强, 贝春华, 余红平, 等. 广西地区人群IL-6及IL-10单核苷酸多态性与HBV相关肝癌关联研究[J]. 中华流行病学杂志, 2011, 32(5):510-513.
QIU X Q, BEI C H, YU H P, et al. Study on the relationship between single-nucleotide polymorphisms in IL-6, IL-10 genes and HBV-related hepatocellular carcinoma[J]. Chinese Journal of Epidemiology, 2011, 32(5):510-513. (in Chinese)
[5] BEK S, NIELSEN J V, BOJESEN A B, et al. Systematic review:Genetic biomarkers associated with anti-TNF treatment response in inflammatory bowel diseases[J]. Aliment Pharmacol Ther, 2016, 44(6):554-567.
[6] BANDAY M Z, BALKHI H M, SAMEER A S, et al. Strong association of interleukin-6-174G/C promoter single nucleotide polymorphism with a decreased risk of colorectal cancer in ethnic Kashmiri population:A case control study[J]. Tumour Biol, 2017, 39(3):1010428317695940.
[7] MAINTINGUER N M, OKI E, FERREIRA CARIOCA A A, et al. Influence of IL1B, IL6 and IL10 gene variants and plasma fatty acid interaction on metabolic syndrome risk in a cross-sectional population-based study[J]. Clin Nutr, 2017, doi:10.1016/j.clnu.2017.02.009. (in Press)
[8] WERNSTEDT I, ERIKSSON A L, BERNDTSSON A, et al. A common polymorphism in the interleukin-6 gene promoter is associated with overweight[J]. Int J Obes Relat Metab Disord, 2004, 28(10):1272-1279.
[9] TODENDI P F, POSSUELO L G, KLINGER E I, et al. Low-grade inflammation markers in children and adolescents:Influence of anthropometric characteristics and CRP and IL6 polymorphisms[J]. Cytokine, 2016, 88:177-183.
[10] BRUCH J, RETTENBERGER G, LEEB T, et al. Mapping of type I loci from human chromosome 7 reveals segments of conserved synteny on pig chromosomes 3, 9, and 18[J]. Cytogenet Cell Genet, 1996, 73(3):164-167.
[11] KELLY D, O'BRIEN J J, MCCRACKEN K J. Effect of creep feeding on the incidence, duration and severity of post-weaning diarrhoea in pigs[J]. Res Vet Sci, 1990, 49(2):223-228.
[12] DEN DUNNEN J T, DALGLEISH R, MAGLOTT D R, et al. HGVS recommendations for the description of sequence variants:2016 Update[J]. Hum Mutat, 2016, 37(6):564-569.
[13] 苗平, 陆梅生, 张冬青. IL-6/IL-6受体与类风湿关节炎关联性研究新进展[J]. 免疫学杂志, 2011, 27(4):355-360.
MIAO P, LU M S, ZHANG D Q. Recent progresses of the association research on IL-6/IL-6R and rheumatoid arthritis[J]. Immunological Journal, 2011, 27(4):355-360. (in Chinese)
[14] 任芳芳. 野生和人工繁育树鼩CD4以及IL-6分子的克隆与分析[D]. 北京:北京协和医学院, 2015.
REN F F. Cloning and analysis of CD4 and IL-6 in the wild and laboratory tree shrews[D]. Beijing:Peking Union Medical College, 2015. (in Chinese)
[15] 王宇昕. IL-6受体与EGF受体相互作用增强IL-6诱导的STAT3信号转导作用研究[D]. 兰州:兰州大学, 2013.
WANG Y X. STAT3 activation in response to IL-6 is prolonged by the binding of IL-6R to EGFR[D]. Lanzhou:Lanzhou University, 2013. (in Chinese)
[16] 王希彪, 李建敏, 许愿, 等. 哺乳仔猪腹泻发生时间和程度的品种间差异及对增重的影响[J]. 东北农业大学学报, 2014, 45(9):79-83.
WANG X B, LI J M, XU Y, et al. Variations between breeds of suckling piglets in diarrhea occurrence time and degree and effect on weight gain[J]. Journal of Northeast Agricultural University, 2014, 45(9):79-83. (in Chinese)
[17] WALLENIUS V, WALLENIUS K, AHRÉN B, et al. Interleukin-6-deficient mice develop mature-onset obesity[J]. Nat Med, 2002, 8(1):75-79.
[18] SZYDLOWSKI M, BUSZKA A, MACKOWSKI M, et al. Polymorphism of genes encoding cytokines IL6 and TNF is associated with pig fatness[J]. Livest Sci, 2011, 136(2):150-156.
[19] BAUMGARTEL K L, GROER M W, COHEN S M, et al. Maternal interleukin genotypes are associated with NICU outcomes among low-birth-weight infants[J]. Biol Res Nurs, 2016, 19(1):36-44.
[20] BOOCKFOR F R, WANG D, LIN T, et al. Interleukin-6 secretion from rat Leydig cells in culture[J]. Endocrinology, 1994, 134(5):2150-2155.
[21] BRYNIARSKI K, SZCZEPANIK M, PTAK M, et al. Modulation of testicular macrophage activity by collagenase[J]. Folia Histochem Cytobiol, 2005, 43(1):37-41.
[22] CUDICINI C, KERCRET H, TOUZALIN A M, et al. Vectorial production of interleukin 1 and interleukin 6 by rat sertoli cells cultured in a dual culture compartment system[J]. Endocrinology, 1997, 138(7):2863-2870.
[23] KERN S, ROBERTSON S A, MAU V J, et al. Cytokine secretion by macrophages in the rat testis[J]. Biol Reprod, 1995, 53(6):1407-1416.
[24] POTASHNIK H, ELHIJA M A, LUNENFELD E, et al. Interleukin-6 expression during normal maturation of the mouse testis[J]. Eur Cytokine Netw, 2005, 16(2):161-165.
[25] RIVAL C, THEAS M S, GUAZZONE V A, et al. Interleukin-6 and IL-6 receptor cell expression in testis of rats with autoimmune orchitis[J]. J Reprod Immunol, 2006, 70(1-2):43-58.
[26] HAKOVIRTA H, SYED V, JÉGOU B, et al. Function of interleukin-6 as an inhibitor of meiotic DNA synthesis in the rat seminiferous epithelium[J]. Mol Cell Endocrinol, 1995, 108(1-2):193-198.
[27] 林文龙. 白细胞介素6与血清生殖激素及精子浓度的相关性分析[J]. 中国计划生育学杂志, 2012, 20(4):250-252.
LIN W L. Correlation analysis of plasma interleukin 6 level with serum reproductive hormones and sperm concentration[J]. Chinese Journal of Family Planning, 2012, 20(4):250-252. (in Chinese)
[28] LAFLAMME J, AKOUM A, LECLERC P. Induction of human sperm capacitation and protein tyrosine phosphorylation by endometrial cells and interleukin-6[J]. Mol Hum Reprod, 2005, 11(2):141-150.

Association Analysis between IL6 Gene Intron 3 Polymorphism and Piglet Diarrhea, Growth and Boar Reproduction Traits

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